| dc.description.abstract | Actinomycetes, a diverse group of Gram-positive bacteria, are renowned for their
filamentous morphology and prolific production of bioactive compounds, including
antibiotics and enzymes. This study focuses on the isolation and characterization of
actinomycetes from the Can Gio Mangrove Biosphere Reserve, a unique ecosystem
recognized for its rich microbial diversity. Sediment and water samples were collected
from four distinct locations within the reserve. The isolation process involved selective
media to promote actinomycete growth, followed by morphological and molecular
characterization. As a result, a total of 20 actinomycete-like isolates were obtained and
confirmed with morphological characterization. Antimicrobial activity was assessed
using the perpendicular streak method against Staphylococcus aureus and Escherichia
coli. Three isolates had activity, among which MT1.12 had strongest inhibitory effects.
Additionally, chitinase testing indicated only one isolate MT4.3 with notable
enzymatic activity. Molecular identification with 16S rRNA gene sequencing revealed
the isolates to be Streptomyces malaysiense, Stenotrophomonas maltophilia, and
Brevibacterium sp. | en_US |
