| dc.description.abstract | Embryonic stem cells (ESCs) hold great potential as a developmental model for
understanding fundamental biological processes and as a potential source of cells for
regenerative therapies. Similarly, the knowledge gained from mouse ESC research can
then be translated to human applications, informing advancements in regenerative
medicine and potential treatments for various conditions. In this research, ESC-like
clusters were derived from 8-cell biopsied blastomeres utilizing PGD-like technique and
subsequently cultured in SC culture medium. The outcomes indicate that isolated
blastomeres from 8-cell embryos possess the capacity to form ESC-like clusters with
similar morphology, which continued to expand over the following 27 days postpassing.
While the results demonstrate the developmental potential of single blastomeres derived
from 8-cell embryos, it is important to acknowledge that the rate of embryonic stem cell
cluster formation remains modest. This indicates that there are certain limitations and
challenges associated with the development of these cells. Further investigation and
exploration are required to better understand the underlying factors influencing the
development of ESCs from single blastomeres. | en_US |
