| dc.description.abstract | Blue eggshells in ducks have long intrigued researchers and farmers due to the
economic benefits of the egg market, especially in Asia. Two G-to-A transitions in the
cis-regulatory region of the ABCG2 gene were proposed to be responsible for the
elevated expression of the ATP-binding cassette superfamily G member 2 (ABCG2)
protein, which transports biliverdin and eventually leads to blue pigmentation of
eggshells. However, no fast and precise methods have been developed to identify duck
individuals with genetic variants causing blue eggshells. In this research, conventional
and multiplex primer-introduced restriction analysis PCR (PIRA-PCR) were utilized
to genotype the blue-eggshell-conferring nucleotide variants by creating synthetic
restriction cut sites for restriction enzymes. The results unveiled two genotypes,
GA/GA and GG/GA, with two previously discovered haplotypes, G-G and A-A, and a
novel haplotype, G-A. The Sanger sequencing confirmed the effectiveness of
multiplex PIRA-PCR, making it a reliable, accurate, and affordable genotyping assay | en_US |
