Characterizing The Immune Response Stimulated By Alpha-toxin Hlah35l/h48l Secreted In Bacillus Subtilis In Mice Using Real-time Pcr

Abstract

S. aureus is a gram-positive bacteria that can cause a variety of life-threatening diseases in humans these days. Since these bacteria colonizes most human bodies, along with the rapid and widespread rate of antibiotic resistance worldwide, making efforts to prevent these bacteria becomes more challenging. Scientists have recently proven that the body's defense against S. aureus is dependent not just on antibody related responses, but also on T-cell-mediated immunity. RT-qPCR is regarded as a quick, accurate, and cost-effective approach for determining the expression of cytokines as biomarkers for T-cell activity at various stages following immunization. In this study, HlaH35L/H48L from B. subtilis secretions was used to examine the immune response of mice for protection against S. aureus via intranasal administration, and its immune responses were characterized via RT-qPCR.