| dc.description.abstract | This study aimed to investigate the response surface methodology (Central
Composite Design) to optimize the extraction conditions of the ultrasound assisted extraction (UAE) of total phenolics, flavonoids, and their antioxidant
capacities from avocado seed (Persea americana Mill.). The study employed a two step optimization approach, where significant factors were identified using One factor-at-a-time method (OFAT) with four factors (ethanol concentration, time,
temperature, and solvent/solid ratio) and the optimal levels of the significant
factors were determined using Central Composite Design. The factors that
significantly affected the extraction yield of total phenolics were found to be
ethanol concentration and temperature. The effects of these two parameters on
the extraction efficiency were evaluated using a response surface methodology.
The results showed that the optimal conditions for the simultaneous extraction of
total phenolics, flavonoids, and antioxidant capacities were 53% ethanol
concentration and ultrasound extraction temperature of 580C. Under these
optimized conditions, the total phenolics, flavonoids, and antioxidant capacities
were 43.31 ± 0.81 mg GAE/g DW, 7.74 ± 0.52 mg RE/g DW and 48.56 ± 1.03
mg AAE/g DW, respectively. The results demonstrate that the integrated
ultrasound-assisted extraction method is an efficient and environmentally friendly
method for the extraction of total phenolics, flavonoids, and antioxidant capacities
from avocado seed | en_US |
