| dc.description.abstract | This study investigated the significance of the processing factors in the ultrasound assisted extraction method for the extraction of polyphenols, flavonoids and their
antioxidant activity from avocado peel (Persea americana Mill.) and subsequently
optimized the process. The study employed a two-step optimization approach, where
a suitable level of significant factors was identified using the one-factor-at-a-time
method with four factors (ethanol concentration, solvent-to-solid ratio, ultrasound
treatment temperature, and sonication time), and the optimal levels of the significant
factors were determined using Box-Behnken Design. In this study, the factors that
significantly affected the extraction yield of total phenolics were found to be ethanol
concentration, ultrasound treatment temperature, and sonication duration. The effects
of these three significant parameters were then chosen to conduct the Box-Behnken
design. The results showed that the optimal conditions for simultaneous extraction of
those three responses were 40.64% ethanol concentration, 60.72oC ultrasound
treatment temperature, and sonication duration of 41.78 min. Under these optimized
conditions, the yields of polyphenols, flavonoids, and their antioxidant activity were
53.21 ± 0.4 mg gallic acid equivalent/g dry basis, 9.60 ± 0.11 mg RE/g dry basis, and
75.20 ± 0.64 mg AAE/g dry basis, respectively. In conclusion, avocado peel is a good
source of bioactive compounds, the ultrasound-assisted extraction method is effective
and ecologically benign for extracting polyphenols and flavonoids, and the Box Behnken design proved to be effective in screening and optimization | en_US |
