| dc.description.abstract | The purpose of this study was to evaluate the effects of ethanol or ethyl acetate on
the crocin extraction from dried gardenia fruit and the physiochemical and bioactive
properties of the extraction. Ultrasound-assisted extraction method was used to
obtain the high yield of crocin from dried gardenia fruit (Gardenia Jasminoides), which
was quantified by using spectrophotometric method, then determine the potential
antioxidant activity by measure the total phenolic content, DPPH-scarvenging effect
(%), and FRAP assay. The highest crocin yield was 1.98 (w:w) corresponding to
mixture of ethanol and water (75:25, v:v) extract, meanwhile the crocin content of
mixture of ethyl acetate and water extract obtained significant lower yield (0.22 %
(w:w)).. The total phenolic content of the sample extracted at optimal condition
(solvent mixture of ethanol and water with sample solvent ratio at 1:32 (w:v), 55oC
for 50 min) was 377.53 mg GAE/100 mL. Antioxidant activity of the gardenia fruit
extract under ultrasonic treatment was investigated based on their ability to scavenge
stable free radicals of 1,1-diphenyl-2-6 picrylhydrazyl (DPPH) and the Ferric Reducing
Antioxidant Power (FRAP) assays that was observed at the threshold of 81.07 %, and
3544.72 µm/L. In general, the result showed a difference value between the sample
with and without the involvement of ultrasonic treatment which was significantly
improved the crocin yield (%), saturated color, crocin content in crude weight (%),
and also enhance the bioactive properties that were total phenolic content, DPPH
scavenging effect (%), and ferric reducing antioxidant power value of the extraction. | en_US |
