In vitro study on antioxidation activities of crude polysaccharide extracted from Amauroderma subresinosum (black Lingzhi)

Abstract

In recent, polysaccharides have been extracted from Ganoderma lucidum (Lingzhi)

by many scientists in China, Japan, and Vietnam for a large number of biological activities such as antioxidant and antitumor activity. The aims of this study were to analyze the quantity and quality of polysaccharides extracted from Ganoderma subresinosum, and evaluates their antioxidant capacity. Crude polysaccharides, water and ethanol extracts were prepared for testing. Crude polysaccharides yield was 2% of dry weight sample, whereas values of ethanol and water extracts were in the range 6.5%, 10.5% of dry weight, respectively. In quality analysis of

polysaccharides, glucose, galactose, and xylose were detected as three major single monosugar in Ganoderma subresinosum by using thin layer chromatography (TLC). In vitro antioxidant activity, free radical scavenging activity using DPPH test and anti-lipid peroxidation effect using malonyl dialdehyde (MDA) test were performed in the range of 50-2000µg/ml of test samples. The results indicated that free radical scavenging activities of test extracts at the 2000µg/ml were equal to that of 0.25-0.5mM ascorbic acid. Similarly, the anti-lipid peroxidation effects of test extract (2000µg/ml) were comparable to that of 5-10 mM Trolox, a soluble form of vitamin E. Thus, this mushroom might be a potential source of bioactive compounds for preventing free radical-related damages.

Keywords:

Ganoderma subresinosum

Polysaccharides

TLC

Antioxidant assays