Isolation and optimization of growing condition of Bacillus spp. isolated from traditional fermented shrimp paste for high fibrinolytic enzyme production

Abstract

Enzymes can be found in nature such as animal, plant, and microorganism sources

in which fibrinolytic enzymes are one of valuable enzymes. Fibrinolytic enzymes have been considered as thrombolytic agents used to treat thrombosis symptoms. Six Vietnamese fermented shrimp pastes were screened for fibrinolytic enzyme activity, of which Thanh Hoa and Ba Tuyet shrimp pastes contained the highest fibrinolytic enzyme activity. Five strains of bacteria were also isolated from Thanh Hoa and Ba Tuyet shrimp pastes and used to produce fibrinolytic enzymes. M2 strain isolated from Thanh Hoa shrimp paste was detected to produce the highest fibrinolytic enzyme activity among five isolated strains and identified as Bacillus weihenstephanensis by 16s rRNA sequencing. In addition, growing conditions of Bacillus weihenstephanensis for producing the highest fibrinolytic enzyme activity were optimized. As a result, 37oC of incubation temperature, 1% of shrimp shell powder (SSP) as a substrate, and 24 hours of incubation time was optimal condition. This study is the first finding on production of fibrinolytic enzyme by Bacillus weihenstephanensis isolated from Vietnamese fermented shrimp paste.

Key words: enzymatic therapy, fibrinolytic enzyme, thrombolytic agent, Bacillus

Weihenstephanensis, specific conditions