Preparation and evaluation the Anona galabra leaf extract-contained formula for burn healing

Abstract

Annona glabra leaves extract which possess substantially antimicrobial activity, however, there is no report on the efficacy of this plant for burn wound healing. Calcium alginate, a natural hemostat capable of forming into hydrogel has a leading position in advanced wound care. This study was aimed at preparing the leaves extract loaded calcium alginate film, and investigating its healing activity on the burn wound lesion. Smooth calcium alginate films containing the leaves extract, in different concentration, were produced. The physicochemical properties and in vivo wound healing profiles of these films were evaluated. Third-degree burn wounds were induced in Swiss albino mice divided into seven groups. Group-I (negative control) received no treatment at all. Groups-II, -III, -IV, -V, and -VI were given formula containing 0%, 1.0%, 2.0%, 3.0%, 4.0% (w/v) leaves extract, respectively. Group-VII (positive control) received the standard dressing, Urgo Algoplaque. Group V received formula containing 3% extract had a significant higher healing rate in comparison with the controls. Healing time in group V was found to be 11.6±0.60 days. Formulas containing Annona glabra leaves extract could facilitate the burn wound healing process and the best leaves extract concentration was found to be 3.0% (w/v).

Key words: Annona glabra Calcium alginate Burn wound Wound healing