The effect of FGF (fibroblast growth factor) on bovine oocyte maturation and the development of bovine embryo after IVF

Abstract

The aim of this study was to describe the requirement of fibroblast growth factor 2 during bovine oocyte maturation and embryo preimplantation development. FGF2 was supplemented at 3 different concentrations (0.5; 5, and 10ng/ml) during in vitro maturation (IVM) and 8h after in vitro fertilization (IVF). Subsequently, the control group was performed without FGF2 supplement. Addition of FGF2 during maturation improved (P ≤0,001) the percentage of oocytes becoming fully mature compared to control (mean ± standard error, 73.915± 1.288; 85.076 ± 1.000; 63.965 ± 1.232 vs 51.194±1.935). The effect of FGF2 on cleavage ratio was detected when 0.5, 5 or 10ng/ml FGF2 was provided during maturation and immediately after fertilization (P ≤0,001). However, there was no significant difference among these 3 treatments (26,643±0,981; 33,868±0,740; and 34,533±1,011 respectively). 5ng/ml FGF2 supplementation significantly influenced (P≤ 0,001) embryo block compared with 0.5 or 10ng/ml (0,582±0,0231 vs 0,290±0,0187 or 0,291±0,0284) while no significant difference was detected between two treatments of 0.5 and 10ng/ml. In conclusion, FGF2 supplementation during maturation and embryo culture was important for optimal oocyte maturation and embryo preimplantation development. Keywords: FGF2 IVM IVF embryo development cleavage ratio embryo block