Determination of alpha - amylase and glucosidase inhibitory of bioactive compounds extracted from defatted sesame meals +CD

Abstract

Sesame meal have been widely used for producing oil technology while its byproduct, defatted sesame meal extracts (DSM) obtained after the removal of oil, presently used as cattle field. Current study evaluates the α-glucosidase, α-amylase inhibition and antioxidant properties of variable sesame cake extracts. Defatted seeds were sequentially extracted with ethanol-water 75:25 (v/v) and methanolwater 75:25 (v/v). Among the extracts results, methanol-water (75:25, v/v) extract illustrated higher antioxidant activities, total phenolic content (TPC), total flavonoid content (TFC) and the α-glucosidase and α-amylase inhibitory potential. Black sesame extracts achieved the highest results in individual experiments compared to other varieties of sesame. This study showed that antioxidant activity of defatted sesame meal extracts was significantly affected by polar solvent, antioxidant assays and extract concentration. These results demonstrate that sesame meal can be used as a source of protein and bioactive compound in the development of functional food for the management of diabetes. Keywords: defatted sesame meal extract; antioxidant activity; phenolic compounds; α-amylase inhibition; α-glucosidase inhibition; functional food; diabetes.