| dc.description.abstract | Polyscias fruticosa L. Harms is a member of the Araliaceae family, which
contains triterpenoid saponins. Because of the amount of saponin triterpen in
Polyscias fruticosa is limited; it is not affordable for the need of pharmaceutical
products. Therefore, callus culture is utilized to produce large quantity of
Polyscias fruticosa in order to meet the demand of the market field and it also
helps lower the price of the products. Thus, this research has been made to
establish the protocol for callus formation and division of Polyscias fruticosa.
Callus culture of Polyscias fruticosa L. Harms were established from leaf
explants. Leaf explants induced callus on Murashige and Skooge (MS) medium
supplemented with 2 mg/l of 2,4-D. Callus division is affected greatly by growth
regulator. Callus after collecting were subculture on MS basal medium
supplemented with various concentrations of 2,4-D, sucrose, coconut water, B1
and Glycine. The highest coefficient of callus division is 9.79 with 1 mg/l of 2,4-D
compare with 0.5, 2 and 3 mg/l. There are no significant differences among the
concentrations of sucrose, coconut water, vitamin B1 and Glycine. However,
according to the morphology and quality of callus which had observed during the
procces, and the saving budget purpose, it had found out that MS medium
supplemented with 2,4-D 1 mg/l, sucrose 30 g/l, Coconut water 10% and
Glycine 10 mg/l were the most suitable medium for efficient callus division.
Keywords: Polyscias fruticosa L. Harms, callus formation, callus division, plant
tissue culture, saponins.
Abbreviations: 2,4-D: Dichlorophenoxyacetic acid; B1: Thiamin HCl; B5
medium: Gamborg medium; Cw: Coconut water; MS medium: Murashige and
Skoog medium | en_US |
