Establishment Of An Optimization Culture Medium For In-Vitro Development Of Mouse Embryos.

Abstract

were a lot of experiments focused on the effect of medium in the development of in-vitro fertilized embryos. However very a little studies on the effect of culture media on the pre-implantation development of in vivo fertilization. Therefore, the aim of this study was to test how difference of two media (CZB and KSOM) on the development of in vivo fertilized 1-cell embryos during pre-implantation development. Female mice (8 to 10 weeks old) were super-ovulated using PMSG/hCG and mated with a proven fertile male of the same strain. Embryos were collected and cultured in vitro around 26 h after hCG injection. Embryo development was evaluated at both 96 h and 120 h after hCG injection. The result showed that the CZB medium was better than the KSOM medium on the development of in vivo fertilized ICR mouse zygote to the expanded blastocyst and hatching blastocyst stage. There should be a concern to use CZB medium for in-vitro culture of mouse embryos. Key words: In vivo fertilization, mouse embryo, in vitro culture, media, CZB and KSOM.