| dc.description.abstract | This study aims at isolation of chemical constituents from Angelica sinensis root and evaluation of their bioactivities. Three isolated compounds were determined as β-sitosterol (375.6 mg), bergapten (50 mg) and β-sitosterol-3-o-β-D-glucopyranoside (184.4 mg). DPPH assay was performed to evaluate antioxidant activity. Ethyl acetate extract and the fractions of 100% methanol (M100) exhibited the highest antioxidant activity among all extracts and fractions, IC50 were 46.20±0.09 μg/mL and 31.57±4.89 μg/mL, respectively). ANS01 compound exhibited antioxidant activity (IC50 = 47.71 ± 1.40 μg/mL). Sulforhodamine B colorimetric assay (SRB assay) was used to evaluate inhibitory activity against MCF-7 and HeLa cells growth. The methanol extract exhibited higher cytotoxicity against HeLa cell line growth at screening concentration (percentage inhibition of 19.77±5.83 % at 100 μg/mL) while n-hexane extract and ethyl acetate extract exhibited higher inhibitory activity against MCF-7 cell growth (percentage inhibition of 15.71±6.41 % and 14.94±2.00 % at 100 μg/mL, respectively). ANS01 compound had potent in anti-breast cancer activity by exhibited inhibitory activity against MCF-7 cell (percentage inhibition of 29.32±2.29 (%) at 50 μg/mL).
Keywords: Angelica sinensis, antioxidant activity, anticancer activity | en_US |
