Study on the antibacterial activity against Pseudomonas aeruginosa of lemon seed extract

Abstract

Pseudomonas aeruginosa (P. aeruginosa) is implicated bacteria which is the almost-universal causes of hospital infection. Moreover, this kind of infection is getting worse because of the resistant rate is higher every passing year. Plenty of scientific evidence suggests that P. aeruginosa is usually treated with the high dosage and easily originate various side effects. Therefore, the search for plant-derived herbs for treatment should be attempted and developed as an alternative solution to current antimicrobial. Among the many plants of natural source, seeds of citrus have received more attention because of high feature about their antioxidant and antimicrobial. Thus, the isolation and successful extraction of phytochemicals from lemon seeds will be medically beneficial. In this study, the antimicrobial efficacy of phytochemicals from lemon seed powder extract solution against the growth and development of Pseudomonas aeruginosa (P. aeruginosa) was thoroughly investigated. Different concentrations of 5% to 15% and 20%, and then cultured in two separate characteristics of media as liquid and solid for testing the colony formation as well as to determine the minimum inhibitory concentration (MIC) against P. aeruginosa. The obtained results of this study have indicated that the extract of lemon seeds showed not only the statistically significant reduction of of P. aeruginosa colony formation in order of 5% to 15% and 20%, but also pointed out the statistically significant reduction of P. aeruginosa colony formation at 10% and 13%, with agar test and broth test in the ratio of 1:4 (v/v) respectively. Beside that this project also contributes to useful information for improving medicinal from solution extracts of lemon seed usage against the growth and development of P. aeruginosa. In summary, the lemon seed extract could effectively inhibit the growth of P. aeruginosa at the selectively applied concentrations. Key words: lemon seed extract, Pseudomonas aeruginosa, antimicrobial