| dc.description.abstract | Pseudomonas aeruginosa (P. aeruginosa) is implicated bacteria which is the
almost-universal causes of hospital infection. Moreover, this kind of infection is getting
worse because of the resistant rate is higher every passing year. Plenty of scientific
evidence suggests that P. aeruginosa is usually treated with the high dosage and easily
originate various side effects. Therefore, the search for plant-derived herbs for treatment
should be attempted and developed as an alternative solution to current antimicrobial.
Among the many plants of natural source, seeds of citrus have received more attention
because of high feature about their antioxidant and antimicrobial. Thus, the isolation and
successful extraction of phytochemicals from lemon seeds will be medically beneficial.
In this study, the antimicrobial efficacy of phytochemicals from lemon seed powder extract
solution against the growth and development of Pseudomonas aeruginosa (P. aeruginosa)
was thoroughly investigated. Different concentrations of 5% to 15% and 20%, and then
cultured in two separate characteristics of media as liquid and solid for testing the colony
formation as well as to determine the minimum inhibitory concentration (MIC) against P.
aeruginosa. The obtained results of this study have indicated that the extract of lemon
seeds showed not only the statistically significant reduction of of P. aeruginosa colony
formation in order of 5% to 15% and 20%, but also pointed out the statistically significant
reduction of P. aeruginosa colony formation at 10% and 13%, with agar test and broth test
in the ratio of 1:4 (v/v) respectively. Beside that this project also contributes to useful
information for improving medicinal from solution extracts of lemon seed usage against
the growth and development of P. aeruginosa.
In summary, the lemon seed extract could effectively inhibit the growth of P. aeruginosa at
the selectively applied concentrations.
Key words: lemon seed extract, Pseudomonas aeruginosa, antimicrobial | en_US |
