Investigation Of Biological Activities Of Crude And Fractionated Extracts Of Musa Balbisiana Colla Fruits

Abstract

Musa balbisiana fruits is an important and commonly used plant for its medicinal and pharmacological properties. The present study aimed to evaluate in vitro antiurolithiatic, antibacterial, and anti-inflammatory activities of crude and fractionated extracts of M. balbisiana fruits. Results showed that crude 45% ethanol extract by hot extraction method had better anti-urolithiasis, antibacterial, and anti-inflammatory activities than crude 45% ethanol extract by cold maceration method and crude water extract by decoction method. Some M. balbisiana fruit extracts had the greater potency in anti-urolithiatic activity compared to positive control. Chloroform fraction had the highest ability against calcium oxalate crystals formation with an IC50 value of 22.04 ± 1.09 µg/mL, which was comparable with sodium citrate (202.08 ± 18.46 µg/mL). In the active in against crystals aggregation, n-hexane fraction had the highest activity with an IC50 value of 414.77 ± 10.85 µg/mL, whereas IC50 value of sodium citrate was 1390.33 ± 92.66 µg/mL. Furthermore, crude and fractionated extracts presented anti-inflammatory activity through inhibition protein denaturation and red blood cells membrane stabilization. Simultaneously, the crude extracts exhibited the ability to against E. coli, P. aeruginosa, and S. aureus. Taken together, the present study had demonstrated that M. balbisiana fruits has properties that support urolithiasis disease.