Contribution To Standardization And Evaluation Of Antioxidant Activity Of Musa Baibisiana Colla Fruit Extract

Abstract

Musa balbisiana (MB) fruits are widespread as an herbal medicine with great therapeutic potential worldwide. The present study was designed to investigate raw materials and crude extract of MB fruits for phytochemicals by qualitative chemical tests and standardization using purity content, chemical characterization, total polyphenol (TPC) and flavonoid (TFC) contents to assess the quality. Besides, antioxidant activity of crude and fractionated extracts of MB fruits was also evaluated using in vitro models including DPPH quenching, ABTS cation decolorization, and reducing antioxidant power assays. Results showed that physicochemical parameters for MB fruits were within prescribed Vietnamese Pharmacopoeia 5th edition limits. Phytochemical analysis of MB fruits revealed the presence of flavonoids, proanthocyanidins, saponins, and triterpenoids. The TPC was 3.92 ± 0.27 mg GAE/g d. w. and 162.64 ± 3.39 mg GAE/g d. w. for the raw materials and crude extract respectively while the TFC was 0.138 ± 0.002 mg QE/g d. w. and 1.222 ± 0.007 mg QE/g d. w.. Furthermore, the crude and fractionated extracts of MB fruits has a high radical scavenging activity. The initial sourcing and quality evaluation of crude extract can be very effective if these preliminary studies are used as raw materials for preparations of herbal formulations. The antioxidant potential of MB fruits can be beneficial for nutritional and medicinal purposes.