Investigation Of The Effect Of Extraction Process On Total Polysaccharides Content From Condonopsis Pilosula

Abstract

Codonopsis pilosula, also known as Dang Sam, has great potential in Vietnam; however, in order to introduce these product to global markets and to domestic consumers believing in Dang Sam’s application, further researches need to be conducted. This study aimed to optimize the processing conditions of each steps of Dang Sam with the highest total polysaccharides content (TPC) and soluble solid content (SSC). The methods used to investigate TPC was Phenol-Sulfuric and SSC was determined by using refractometer. The drying conditions were identified using four drying temperatures (60, 70, 80 and 90oC) and four ethanol concentrations (75, 80, 85 and 90 %). The sample was then examined with 4 different ratios between material and solvent (1:20, 1:25, 1:30, 1:35), temperatures of extraction ( 75, 80, 85, 90oC) and times of extraction (1.5, 2, 2.5, 3 hours). As a result, the study indicated the optimal drying conditions of Dang Sam was dried at 80oC for 7 hours, the optimal ethanol concentration was 75% which extracted at 75oC in 3 hours was higher than those extracted from others. At this condition, the highest TPC percentage of the extract was 8.12 ± 0.03%. In general, this study contributed greatly to food industry with the updates of polysaccharides content, nutritional value and processing conditions of Dang Sam. This project will provide a useful extraction method for further studies which improve the TPC, nutritional value applied in food industry and science chemical-rich extract for developing a functional food.