Extraction of oil from H'mong pumpkin seed (Cucurbita ficifolia) using enzyme - assisted three phase partitioning and evaluation of the bioactive compounds of the final oil

Abstract

The aim of this study was to investigate the effects (enzyme concentration, incubation temperature and time) of two individual enzymes (cellulase or protease) on the yield of oil from H’mong pumpkin seeds by using enzyme-assisted three-phase partitioning extraction (EATPP). For the Cellulase, the highest oil yield was obtained as 37.50 ± 0.50 % in the conditions of 0.5%, at 40 °C within 2h of incubation; for protease, it was 41.00 ± 0.50 %, achieved in the conditions of 1.5%, at 50°C within 3h of incubation. The quality of final oil such as peroxide value (4.73 ± 0.15 meq O2/kg) and free fatty acid (1.45 ± 0.07%) from cellulase (EATPP). The oil obtained from EATPP was significantly lower than Soxhlet; howerver, significantlt higher than TPP. Both peroxide value and free fatty acid obtained by EATPP (cellulase) were lower than that by EATPP (protease), which were lower than Soxhlet method as well. Likewise, TPC, TFC and DPPH of oil obtained by EATPP were significantly higher than these bioactive compounds presented in oil extracted by Soxhlet method. The enzyme-assisted three-phase partitioning extraction of H’mong pumpkin seed oil was efficient extraction method which increasing oil yield compared to TPP and quality of oil.