| dc.description.abstract | The aim of this study was to investigate the effects (enzyme concentration,
incubation temperature and time) of two individual enzymes (cellulase or
protease) on the yield of oil from H’mong pumpkin seeds by using enzyme-assisted
three-phase partitioning extraction (EATPP). For the Cellulase, the highest oil yield
was obtained as 37.50 ± 0.50 % in the conditions of 0.5%, at 40 °C within 2h of
incubation; for protease, it was 41.00 ± 0.50 %, achieved in the conditions of
1.5%, at 50°C within 3h of incubation. The quality of final oil such as peroxide
value (4.73 ± 0.15 meq O2/kg) and free fatty acid (1.45 ± 0.07%) from cellulase
(EATPP). The oil obtained from EATPP was significantly lower than Soxhlet;
howerver, significantlt higher than TPP. Both peroxide value and free fatty acid
obtained by EATPP (cellulase) were lower than that by EATPP (protease), which
were lower than Soxhlet method as well. Likewise, TPC, TFC and DPPH of oil
obtained by EATPP were significantly higher than these bioactive compounds
presented in oil extracted by Soxhlet method. The enzyme-assisted three-phase
partitioning extraction of H’mong pumpkin seed oil was efficient extraction method
which increasing oil yield compared to TPP and quality of oil. | en_US |
