Application Of Clove And Cinnamon Oil In Acne Treatment

Abstract

Since ancient times, cinnamon and clove essential oils have been known as stable antibacterial agents containing components such as eugenol, cinnamaldehyde and other important compounds. Besides, with benign plant origin, cinnamon and clove extracts also possess redox properties responsible for antioxidant activity. After being extracted from powder, essential oils were analyzed by GC/MS for the results of 56.40% of eugenol in clove and 92.28% of trans-Cinnamaldehyde in cinnamon. Their antioxidant activity was evaluated based on the results of TPC and DPPH scavenging activity. The results of TPC were 368.2069 ± 1.1945 (mg GAE/g powder extract) for clove and 26.82759 ± 1.1945 (mg GAE/g powder extract) for cinnamon. The IC50 value of clove and cinnamon were 205.4605 (uL/mL) and 257.2773 (uL/mL), respectively. Most importantly, with the aim of applying the antibacterial properties of cinnamon and clove in the acne treatment, an in vitro evaluation was performed on Propionibacterium acnes using the broth microdilution method and obtained impressive MIC results wind 0.5% (v/v) for clove and 0.625% (v/v) for cinnamon. To control the amount of essential oils used as well as expand the application of the natural compounds, full extraction of cinnamon and clove essential oils will continue to be loaded in the nanoemulsion system using PIT the method. The nanoemulsions were finally optimized with 20% of 7:3 mixture of surfactants Tween 80: Span 80, Caprylic/capric triglyceride, and distilled water to find out the affection of surfactant proportion on the stability of the products. The results after one month showed that, the higher concentration of emulsifier, the smaller size of nanoemulsion. Nanoemulsions loaded with the essential oils were around 100 nm in size, with PDI (under 0.2) and Zeta (-18mV to -30mV) for stability through one month. Furthermore, nanoesmulsion loaded with the essential oils were tested the antimicrobial activities again to confirm their abilities still working through the processes.