Evaluation Acute And Sub-Acute Oral Toxicity Of Hydroalcoholic Extract Of Ensete Glaucum Seeds In Mice

Abstract

Ensete glaucum (E. glaucum) is a valuable and widely used plant due to its medicinal and pharmacological properties. The aim of this study is to evaluate acute and sub-acute oral toxicity of hydroalcoholic extract of E. glaucum seeds in Swiss-albino mice. For acute oral toxicity study, mice in both genders were received the hydroalcoholic extract (dosage 0.5 mL/10 g BW), were monitored for behavior and mortality within 72 hours. For sub-acute oral toxicity study, mice in both genders were received the hydroalcoholic extract (dosage 6 mL/kg BW) for 56 days. During experiment, body weight of the mice and clinical observation was recorded, biochemical parameters and hematological parameters were determined before the test, day 28th and day 56th. For the results, the hydroalcoholic extract from E. glaucum seeds has acute toxicity on white mice with Swiss albino strain in both sexes with LD50 of 17.46 mL/kg. For sub-acute toxicity test, the results showed that liver, kidney, and heart histology identify some morphological changes. Furthermore, hematological, and biochemical data demonstrated that the extract had slight effects on some parameters on mice. As a result,hydroalcoholic extract from E. glaucumseeds shows a slight effect on mice.In conclusion, the hydroalcoholic extract showed acute toxicity and a minor but insignificant effect on biochemical, hematological, and internal organ parameters in sub-acute oral toxicityon mice after two months of research.