| dc.description.abstract | Ilex genus has been illustrating for possessing numerous medicinal treatments
such as antioxidant, anti-inflammation, anti-obesity, anti-diabete, and willing to work
as an antimicrobial source. The present study aims to suggest the potential
pharmaceutical activities of Ilex cochinchinensis and Ilex annamensis which are still
rare and lack acknowledgement. To select the extraction solvents that give the most
effective bioactivity test results, several solvents were examined consisting of
methanol (MeOH), methanol and water with the ratio 1:1 (M/W), and water (W)
combining with maceration method. Additionally, all of them were screened for 6
bioactivities: lipase, α-glucosidase, α-amylase, and tyrosinase inhibition activity as
well as antioxidant, and antibacterial ability. The leaf of both I. annamensis and I.
cochinchinensis, and the branch of I. annamensis in water solvent exhibited the
extremely high ability amongst 12 samples that gave over 90% in limiting the
glucosidase activity, resulting in the IC50 = 21.33 ± 0.94, 27.98 ± 1.81, and 25.47 ±
0.25 ppm, respectively, which was very close to the positive control (IC50 = 16.08 ±
0.05 ppm). They were considered as the potential to produce natural α-glucosidase
inhibitors. Our project may provide new natural sources for the treatment of diabetes
since, besides the effect on glucosidase, our samples also express the moderate
inhibitory capacity against α-amylase. Moreover, there were 2 samples that could be
considered as the new herbal source for tyrosinase inhibitors which were the leaf of I.
cochinchinensis with IC50 = 65.29 ± 0.99 ppm, and the branch sample of I. annamensis
with IC50 = 59.90 ± 1.89 ppm. Nonetheless, for the remaining bioactivities, the samples
seemed to express lower ability compared to their positive controls. Proof that all the
sample extracts revealed the ability in limiting the oxidation process under 50%.
Furthermore, excepting P. aeruginosa with the moderate ability that the plant extracts
were resistant to, S. aureus and E. coli were recorded with very poor or no resistance
activity. | en_US |
