CHARACTERIZATION OF MULTI-DRUG RESISTANCE SALMONELLA ENTERICA SEROTYPE KENTUCKY 1066

Abstract

Salmonella is one of the major food-borne in worldwide. It accounts for around 25% of all diarrheic infectious disease in humans. There are more than 25000 different Salmonella enterica and most of them are dangerous. With the rise of multi-drug resistance, it becomes harder to treat or prevent Salmonella. This study aims to provide the basic data about multi – drug resistant in Salmonella enterica serotype kentucky 1066 based on whole genome sequencing analysis and plasmid characterization. WGS is used to predict antibiotic resistance gene in the genome of S. kentucky 1066. Moreover, via WGS analysis, a great number of antibiotic resistance genes in Salmonella Kentucky 1066 was predicted. Contig 25 and 29 were picked to analysis because time limitation.Alkaline lysis method is used for plasmids extraction in S. kentucky 1066 and transformed E. coli JM109 for verify what plasmid contain antibiotic. Gel electrophoresis is performed for checking plasmid extraction. Using disk diffusion method in antibiotic susceptibility test, for the determination of the multi – drug resistant phenotype of S. kentucky 1066 and what antibiotic resistance gene in plasmid in transformed E. coli. The test was done with five different kinds of antibiotic. S. kentucky 1066 was recorded to resist Ampicillin (AMP), Amoxicillin (AMO), Ciprofloxacin (CIP), Tetracycline (TET), and Gentamicin (GEN). Besides that, difference clone of transformed E. coli uptook the plasmid differently that takes the advantage to characterize antibiotic resistance and help for plasmid determination for molecular and pharmaceutical sciences.