| dc.description.abstract | Salmonella is one of the major food-borne in worldwide. It accounts for around 25% of
all diarrheic infectious disease in humans. There are more than 25000 different Salmonella
enterica and most of them are dangerous. With the rise of multi-drug resistance, it
becomes harder to treat or prevent Salmonella. This study aims to provide the basic data
about multi – drug resistant in Salmonella enterica serotype kentucky 1066 based on whole
genome sequencing analysis and plasmid characterization. WGS is used to predict
antibiotic resistance gene in the genome of S. kentucky 1066. Moreover, via WGS
analysis, a great number of antibiotic resistance genes in Salmonella Kentucky 1066 was
predicted. Contig 25 and 29 were picked to analysis because time limitation.Alkaline lysis
method is used for plasmids extraction in S. kentucky 1066 and transformed E. coli JM109
for verify what plasmid contain antibiotic. Gel electrophoresis is performed for checking
plasmid extraction. Using disk diffusion method in antibiotic susceptibility test, for the
determination of the multi – drug resistant phenotype of S. kentucky 1066 and what
antibiotic resistance gene in plasmid in transformed E. coli. The test was done with five
different kinds of antibiotic. S. kentucky 1066 was recorded to resist Ampicillin (AMP),
Amoxicillin (AMO), Ciprofloxacin (CIP), Tetracycline (TET), and Gentamicin (GEN).
Besides that, difference clone of transformed E. coli uptook the plasmid differently that
takes the advantage to characterize antibiotic resistance and help for plasmid
determination for molecular and pharmaceutical sciences. | en_US |
