Evaluation Of In Vitro Anti-Inflammatory And Anti-Bacterial Potentiality Of Dioscorea SP. Roots

Abstract

Dioscorea hamitlonii Hook. f. is scientific name also called as Dioscorea persimilis Prain & Burkill or Hoai Son in Vietnamese. In addition to being consumed as staple food and sweet desert soup by native ethnic minorities, Dioscorea hamitonii also being used for the medical purposes with numerous bioactivities it showed. The extractions of Dioscorea hamiltonii were performed using different Dioscorea hamitlonii tubers collected from several regions in Vietnam with deionized water as solvent. This study was conducted in order to obtain and evaluate the anti-bacterial and anti-inflammatory potential of Dioscorea hamiltonii commercial samples extracted by water with maceration method. In this study, total polyphenol content (TPC) of the water extracts were determined by Folin-Ciocalteu method while total flavonoid content (TFC) of the water extracts were quantified by Aluminum chloride method. In this study, biological properties of Dioscorea hamiltonii water extracts were evaluated for anti bacterial activity by agar well diffusion method against bacterial strains namely Micrococcus luteus (M. luteus), Staphylococcus aureus (S. aureus), Pseudomonas aeruginosa (P. aeruginosa) and Klebsiella pneumoniae (K. pneumoniae). Beside, anti inflammatory effect of D. hamiltonii extracts were also evaluated by egg albumin denaturation method and membrane stabilization assay when there was no in-vitro study about this characteristic of this plant. Particularly, the TPC of water extract of BMf, BMfd, BMd and KTd samples were 0.12 mg GAE/g, 0.24 mg GAE/g, 0.405 mg GAE/g and 0.295 mg GAE/g, respectively. The result for TFC of four extract samples were also reported from 0.074 mg NE/g to 0.275 mg NE/g. Anti-bacterial activity of the extracts were determined at concentration of 500 mg/ml and BMf sample gave the highest inhibition to M. luteus (11.4 ± 0.053mm). The protection of egg albumin denaturation of BMd sample was 62.71% at concentration of 800 ug/ml and protection of RBC membrane from hemolysis of BMd extract at dose of 1000 ug/ml was 34.508%. Therefore, the biological activities of the extracts originated from the different sources indicated it is important to select the Dioscorea hamiltonii for functional and pharmaceutical field