Initial Study On Bioactivities Of Fruiting Body Extracts Of Fomes Fomentarius And Trametes Versicolor

Abstract

The purpose of this study was to investigate antioxidant and neuroprotective activities of ethanol extracts prepared from fruiting bodies of Fomes fomentarius and Trametes versicolor. The results show that all the extracts displayed antioxidant activities, and the activities are dose-dependent. Through DPPH method, the highest antioxidant capability was observed with 70% EtOH F. fomentarius extract (IC50 of 53.12 ± 2.32 µg/mL), followed by 90% EtOH F. fomentarius extract (IC50 of 113.88 ± 16.89µg/mL), 90% EtOH T. versicolor extract (IC50 of 229.61 ± 16.04 µg/mL), and 70% EtOH T. versicolor extract (IC50 of 528.63 ± 11.35 µg/mL). These figures indicated that antioxidant activity significantly varies by species and type of solvent used. Regarding cellular protection against Hydrogen Peroxide (H2O2) and 1-Methyl-4-phenyl 1,2,3,6-tetrahydropyridine (MPTP), F. fomentarius extracts showed significant ability to protect cells against the toxins, whereas T. versicolor extract only demonstrated moderate activity based on MTT assay. When cells were exposed to H2O2 alone, 78.67% cell population remained viable. However, treatment supplied with EtOH 90% F. fomentarius extract, EtOH 70% F. fomentarius extract, and EtOH 70% T. versicolor extract could enhance the viability to 92.67%, 92.00%, and 81.67%, respectively. EtOH 90% T. versicolor extract seemed not to be able to protect cells well as the viability of that treatment was 68.33%. When cells were exposed to MPTP neurotoxin, the highest viability belonged to the treatment with EtOH 70% F. fomentarius extract (90.33%), followed by EtOH 90% F. fomentarius extract (89.67%), and EtOH 70% T. versicolor extract (81.67%). These are significantly higher than the negative control (73.33%). However, EtOH 90% T. versicolor extract (67.67%), showed nonsignificant protection properties. Fomes fomentarius ethanol extracts are potential for neuroprotective research and worth further study