| dc.description.abstract | The purpose of this study was to investigate antioxidant and neuroprotective activities of
ethanol extracts prepared from fruiting bodies of Fomes fomentarius and Trametes
versicolor.
The results show that all the extracts displayed antioxidant activities, and the activities are
dose-dependent. Through DPPH method, the highest antioxidant capability was observed
with 70% EtOH F. fomentarius extract (IC50 of 53.12 ± 2.32 µg/mL), followed by 90%
EtOH F. fomentarius extract (IC50 of 113.88 ± 16.89µg/mL), 90% EtOH T. versicolor
extract (IC50 of 229.61 ± 16.04 µg/mL), and 70% EtOH T. versicolor extract (IC50 of
528.63 ± 11.35 µg/mL). These figures indicated that antioxidant activity significantly
varies by species and type of solvent used.
Regarding cellular protection against Hydrogen Peroxide (H2O2) and 1-Methyl-4-phenyl 1,2,3,6-tetrahydropyridine (MPTP), F. fomentarius extracts showed significant ability to
protect cells against the toxins, whereas T. versicolor extract only demonstrated moderate
activity based on MTT assay.
When cells were exposed to H2O2 alone, 78.67% cell population remained viable.
However, treatment supplied with EtOH 90% F. fomentarius extract, EtOH 70% F.
fomentarius extract, and EtOH 70% T. versicolor extract could enhance the viability to
92.67%, 92.00%, and 81.67%, respectively. EtOH 90% T. versicolor extract seemed not
to be able to protect cells well as the viability of that treatment was 68.33%.
When cells were exposed to MPTP neurotoxin, the highest viability belonged to the
treatment with EtOH 70% F. fomentarius extract (90.33%), followed by EtOH 90% F.
fomentarius extract (89.67%), and EtOH 70% T. versicolor extract (81.67%). These are
significantly higher than the negative control (73.33%). However, EtOH 90% T.
versicolor extract (67.67%), showed nonsignificant protection properties.
Fomes fomentarius ethanol extracts are potential for neuroprotective research and worth
further study | en_US |
