Comparison Of The Gene Expression Profiles For Reproductive Trait In Wildtype And Captive Populations Of Female Black Tiger Shrimp (Penaeus Monodon) Using RNA-SEQ Approach

Abstract

The black tiger shrimp Penaeus monodon is a significant aquaculture species in many countries,especially in Vietnam. The reproductive success of this economically important species largely depends on its ability to reproduce successfully in captivity. However, captive populations often exhibit reduced reproductive performance compared to their wild counterparts. In this study, the aim was to compare the gene expression profiles related to reproductive traits in female black tiger shrimp between wildtype and domesticated populations using RNA-Sequencing. Altogether, 27,127,202 (97.5%) and 25,976,434 (93.9%) clean reads were generated for the captive and wildtype P. monodon, respectively.De novo assembly produced totally 81,820 contigs with an average length of 734 bp and N50 of 1,542 bp. There were 7,373 unigenes with differential expression between the ovaries of wildtype and captive P. monodon, in which 3,664 genes were up regulated and3,048 genes were down-regulated, respectively. The results confirmed that vitellogenin, hemolymph clottable, clip domain serine proteinase 1 and serine proteinase were up- regulated in domesticated P. monodon, while, thrombospondin, glutamine synthetase, prostaglandin, peritrophin and cytosolic phospholipase were down-regulated. In addition,Gene Ontology (GO) concepts were used to further enrich the differentially expressed transcripts. There were 20,371 contigs assigned with GO terms. This study created a transcriptome data to enhance the reproductive efficiency of black tiger shrimp by the breeding programs in the future.