| dc.description.abstract | The black tiger shrimp Penaeus monodon is a significant aquaculture species in many
countries,especially in Vietnam. The reproductive success of this economically important
species largely depends on its ability to reproduce successfully in captivity. However,
captive populations often exhibit reduced reproductive performance compared to their
wild counterparts. In this study, the aim was to compare the gene expression profiles
related to reproductive traits in female black tiger shrimp between wildtype and
domesticated populations using RNA-Sequencing. Altogether, 27,127,202 (97.5%) and
25,976,434 (93.9%) clean reads were generated for the captive and wildtype P. monodon,
respectively.De novo assembly produced totally 81,820 contigs with an average length of
734 bp and N50 of 1,542 bp. There were 7,373 unigenes with differential expression
between the ovaries of wildtype and captive P. monodon, in which 3,664 genes were up regulated and3,048 genes were down-regulated, respectively. The results confirmed that
vitellogenin, hemolymph clottable, clip domain serine proteinase 1 and serine proteinase
were up- regulated in domesticated P. monodon, while, thrombospondin, glutamine
synthetase, prostaglandin, peritrophin and cytosolic phospholipase were down-regulated.
In addition,Gene Ontology (GO) concepts were used to further enrich the differentially
expressed transcripts. There were 20,371 contigs assigned with GO terms. This study
created a transcriptome data to enhance the reproductive efficiency of black tiger shrimp
by the breeding programs in the future. | en_US |
