Comparison Of Antioxidant Ability Between Extracts From Agaricus Bisporus And Ganoderma Lucidum For Cosmetic Application

Abstract

Mushrooms belong to the fungi family and have a variety of secondary metabolites, including various phenolic compounds and bioactive activities that act as strong antioxidants. The purpose of the present study was to compare antioxidant activities, total phenolics, and total flavonoid content between extracts of Agaricus bisporus (white button mushroom) and Ganoderma lucidum (reishi mushroom), as well as to evaluate the antioxidant activities of a facial serum containing these extracts. All mushroom samples were extracted with 95% ethanol at room temperature for 3 days, and extracts stored at 4 degrees were determined at IC50 concentrations using the DPPH and ABTS assays. The phenolic compound analysis was performed using the Folin-Ciocalteu reagent, and the flavonoid content was quantified using the reaction with aluminum chloride salt. Additionally, extracts were used to formulate facial serums, and their antioxidant abilities were compared with those of commercially available antioxidant serums. As a result, Ganoderma lucidum extract exhibited the strongest antioxidant capacity among the three extracts, with IC50 concentrations by DPPH (0.309 ± 0.06 mg/ml) and ABTS (3.978 ± 0.098 mg/ml), total phenolic content (17.24 ± 0.55 mg/ml), and total flavonoid content (31.12 ± 0.3 mg/ml). Next, the live extract of Agaricus bisporus showed IC50 concentrations by DPPH (1.267 ± 0.07 mg/ml) and ABTS (5.937 ± 0.622 mg/ml), total phenolic content (13.65 ± 0.86 mg/ml), and total flavonoid content (9.69 ± 0.49 mg/ml). Finally, the cooked extract of Agaricus bisporus had the lowest antioxidant activities of the three extracts, with IC50 concentrations of DPPH (2.796 ± 0.20 mg/ml) and ABTS (7.393 ± 0.30 mg/ml), total phenolic content (9.89 ± 0.57 mg/ml), and total flavonoid content (9.9 ± 0.63 mg/ml). Based on the results, Ganoderma lucidum extract was the highest antioxidant; however, the antioxidant properties exhibited by the extract of the live Agaricus bisporus mushroom could be considered for application in cosmetics.