Evaluation of pharmacological activity of flavonoids in Euphorbia Thymifolia Linn

Abstract

This study investigates antioxidant and antidiabetic activity of ethyl acetate extract of Euphorbia Thymifolia Linn and two flavonoids, Luteonin (EL01) and quercetin-3-O- arabinofuranoside (EL02), were purified and identified from ethyl acetate extract. The antioxidant potential was evaluated by 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay. The antidiabetic activity was analyzed by inhibiting the α- glucosidase activity. Total phenolic and flavonoid content was also determined. The EL02 showed a strong ingredient of antioxidants with IC50 of 0.013±0.50 µg/mL that is higher than Ascorbic acid with IC50 of 0.097±0.29 µg/mL. The EL02 also had a potential in antidiabetic activity with IC50 of 11.76±0.18 µg/mL, whereas IC50 of positive control, Acarbose, was 250.4±0.014 µg/mL. Key words: Euphorbia Thymifolia Linn, antioxidant activity, antidiabetic activity, Luteonin, Quercetin-3-O-arabinofuranoside